Phytochemical analysis of traditional medicinal plants and. The plant leaves are very good nutrient supplement for malnutrition and also used as an antibiotic. Analysis of the essential oils gcms analysis of the essential oil was carried out on an agilent. The seed oil is widely employed in the indian subcontinent, and the leaves seem to have a lower relevance, but the ethnobotanical information of bali indonesia considers the utilisation of leaves. Phytochemical analysis of plant extracts table 1 presents the result of the phytochemical screening of the stem bark of azadirachta indica. Phytochemical analysis on moringa oleifera and azadrichta. Qualitative phytochemical analysis of methanolic leaf extract of some selected plant species. Numerous research groups have also reported such studies throughout the world 48. This study investigated the phytoconstituents in neem seed and flower, jatropha curcas stem and root bark and grape stem bark and leaves in some selected solvents. Extraction of phytochemicals and study of indian j.
Analysis of components of neem azadirachta indica oil by. Agarwood is a highly valuable fragrant resinous wood and has been listed as endangered species in recent years. This research work was focus on determining the quantitative phytochemical analysis and antifungal susceptibility of azardirachta indica against some strains of candida albicans. Preliminary phytochemical screening of petroleum ether and alcohol extracts of symplocos racemosa was carried out by davmurari 2010. Aqueous extracts of azadirachta indica neem was subjected to in vitro antibacterial assay against human pathogenic escherichia coli and salmonella sp by cup diffusion method. Quantitative phytochemical analysis and antifungal. Phytochemical screening, quantitative estimates of.
Phytochemical characterization, in vitro antibacterial. Phytochemical analysis and antibacterial evaluation of. Curry leaves, botanical name murrayakoenigii, spices m. In folklore and ayurvedic all parts of the tree used in different healing procedures for different diseases. The leaves of azardirachta indica was susccessively extracted using reflux method of extraction. Wadood a, ghufran m, jamal sb, naeem m, khan a, et al. Analysis of phytochemical variability in neem formulations. The phytochemical analysis of the extract of azadirachta indica leaves for alkaloids, saponins, tannins, glycosides, anthraquinones, terpenes, and flavonoids was carried out using the methods described by harborne 1973. Phytochemical analysis preliminary phytochemical analysis for leaves l. Vaishnav college, chennai 600 106, tamil nadu received 11 august 2009. Keywords phytochemical, qualitative, moringa concanensis accepted. Preliminary phytochemical screening was performed using various biochemical tests. Phytochemical, antimicrobial activity, murrayakoenigii.
The present study was undertaken to prepare crude extracts of neem leaves with different polarity organic solvents by using a maceration method and assessing phytochemical screening, the total phenol content and antioxidant activity by the spectroscopic method. Owing to the significance in the above context, such preliminary phytochemical screening of plants is the need of the hour in order to discover and develop novel therapeutic agents with improved efficacy. Extraction was carried out in 700ml distilled water at 60oc for 8 hours using the soxhlet extractor. These phytochemicals are derived from various parts of plants such as leaves, flowers, seeds, barks, roots and pulps. Studies on extraction and hplc analysis of azadirachtin. The present study was designed to evaluate the phytochemical screening, antimicrobial and antioxidant activities of anabasis aphylla l. Phytochemical screening of the leaf extract of leaves both fresh and shade dried indicated the presence of flavonoids, terpenoids, tannins, alkaloids, steroids and glycosides. The leaves were air dried under shade and hand crushed to obtain a 2. Phy to chemical screening of azadirachta indica neem.
Phytochemical screening and antimicrobial activity of some. Phytochemical screening revealed the presence of alkaloid, flavonoid, saponin, terpenoid, steroid and sterols in the extracts of aerial parts of a. Phytochemical screening revealed the presence of bioactive compound saponin in all the parts of the three plants in water extract only. The phytochemical analysis will be carried out only on the leaves of the azadirachtaindica neem leaves and limited to the study of the following tannin, saponin, flavonoid and alkaloid. Phytochemical analysis of medicinal plants occurring in. The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the e. The result of these analyses of leaves of medicinal plants shows that carbohydrates, alkaloids, glycosides, phenols and flavonoids are present in leaves of calotropis procera. For volatile oil estimation 50 mg of powdered material crude drug is taken and subjected to hydro distillation. Phytochemical tests were performed and showed that the antibacterial activity of plant azadirachta indica leaves was due to the presence of phytochemical compounds like reducing sugar, glycosides, tannins, triterpenes. Plant materials the leaves and fruits of azadirachta indica melia and. The phytochemical analysis and antibacterial activity of ocimum gratissimum scent leaf and vernonia amygdalina bitter leaf extracts was carried and it showed that the extracts had antibacterial effects on some drug resistant bacteria. The results of the phytochemical screening of spondias mombin and azadirachta indica is summarized in table1.
Google scholar the hexane extract yielded six fatty acids and six unsaponified compounds resolved by high speed countercurrent chromatography hsccc. Phytochemical analysis shows the presence of flavonoids, tannins triterpenoids, saponins, sterols, alkaloids and carbohydrates. Preliminary phytochemical screening, quantitative analysis. Phytochemical analysis and antimicrobial activity of methanolic, ethanolic and acetonic extracts of stem bark and leaf of neem plant azadirachta indicaeffiong edet bassey 1, gwana adamu mohammed 2, halima mohammed bala 3, umeh sophina ogonna 1, bagudu buhari yawuri 4, okoli c. Neem leaves were first found in mohenjodaro, pakistan puri, 2005 6. Micromorphological and preliminary phytochemical studies. Medicinal plant, andrographis affinis, phytochemical analysis, antimicrobial activity. Phytochemical analysis of methanolic leaf extracts of azadirachta indica has shown the presence of biological compounds like, alkaloids, flavonoids, saponins, etc which are then compared to aqueous leaf extracts of the plant. Phytochemical analysis and antibacterial activity of. Phytochemical analysis and antimicrobial activity of. Neem leaves has antibacterial properties and could be used for controlling airborne bacterial contamination in the residential premise.
Characterization of the chemical state of iron in the leaves of wild. Quantitative analysis were also conducted to determine the amount of alkaloids, flavonoids, phenol and carbohydrate. Pdf phytochemical analysis and antibacterial properties. Phytochemical analysis of methanolic extracts of leaves of. Qualitative phytochemical analysis the crude powder of leaf and stem of t. Biological activities of different neem leaf crude. The present study was undertaken to prepare crude extracts of neem leaves with different polarity organic solvents by using a maceration method and assessing phytochemical screening, the total. Qualitative and quantitative phytochemical analysis of. Invitro antimicrobial activity and phytochemical analysis. Studies on extraction and hplc analysis of azadirachtin from kernels of neem seeds and quality assurance, school of introduction the botanical name of the neem is azadirachta indica and it belongs to the family meliaceae. Ms method was established to rapidly identify authenticity of agarwood without sample preparation, some 2. The leaves powder was successively extracted with petroleum ether, chloroform, ethanol, water, hydroalcoholic. It indicated that phytochemical screening of the ethanolic extract of a. Moringaceae is a very useful tree in tropical countries.
Focus on phytochemical pesticides, the neem tree edited by. Phytochemical screening and antimicrobial activity of. Steroids, anthroquinone, tannin, oils and resins were absent in the extract. The phytochemical and proximate analyses of psidium guajava leaves were determined using the methods of association of official analytical chemists a.
Meliaceae is widely known for its cold pressed seed oil, mainly used as insecticide, but also for cosmetic, medicinal and agricultural uses. Analysis of phytochemical variability in neem formulations s gunasekaran 1 and b anita 2 1periyar univeristy, salem 636 011, tamil nadu, india 2department of physics, d. Phytochemical analysis of the extracts revealed the presence of glycosides, alkaloids, oils, sapponins and flavanoids. Abstract the present investigation deals with the phytochemical studies of leaves of different medicinal plants like andrographis. The method used was gas chromatography coupled with mass spectrometry. Antioxidant activity was analyzed by using 2,2diphenyl1picrylhydrazylhydrate assay. Studies were conducted to determine the phytochemicasl present in moringa olrifera and azadrichta indica leaves.
The plant leaves were effective against all the tested organisms. The study suggest further research regarding the pharmacological investigations of this plant. General techniques involved in phytochemical analysis k. Methanol extract of azadirachta indica, phytochemical analysis, antibacterial activity. Phytochemical screening the phytochemical analysis of the extract of azadirachta indica for the determination of alkaloids, saponins. Fresh matured neem plant leaves were obtained from the university of maiduguri campus and identified by a botanist. To evaluate the antibacterial activity of moringa oleifera leaf extracts against four.
Neem azadirachta indica is widely used in the ayurvedic medicine system for treating malaria and fever. Curry leaves also known as karieppilai, karipatta, sweet neem leaves and kadipattta etc. The fingerprint profiles of methanol extract and ethyl acetate sub fraction of azadirachta indica leaves were conducted using preliminary phytochemical screening and high performance thin layer chromatography hptlc analysis. Phytochemical analysis of some nigeria medicinal plants.
Extraction the dried powder of the leaf and stem of t. Analysis of variance showed significant difference between zone diameters of chlorhexidine, neem leaf extract, and 3% sodium hypochlorite against e. Studies of antimicrobial properties of different leaf. The quantitative analyses showed the concentrations mg100g of the phytochemical constituents as flavonoids 0. Minimum bactericidal concentration mbc value of 5mgl was obtained against escherichia coli and salmonella sp were found to. The phytochemicals, minerals and vitamins a and c compositions of spondias mombin leaves were determined by igwe et al. The percentage phytochemical composition of the leaves of pawpaw, bitter kola, tetrapleura, neem and ginger was investigated using the methods of the association of analytical chemists, harbone and the alkaline picrate method.
Pdf preliminary phytochemical analysis of hibiscus. Thin layer chromatography and bioautography agar overlay assay of ethanol extracts of neem, tulsi and aloe vera indicated flavonoids and tannins as major active compounds against methicillinresistant staphylococcus aureus. Prasad et al 7 have explained antibacterial, phytochemical and antioxidant potential of some ocimum species. General techniques involved in phytochemical analysis international journal of advanced research in chemical science ijarcs page 29 11. Sahira banu assistant professor, department of chemistry. Qualitative phytochemical screening was carried out using the crude leaf extracts in three different solvents such as water, alcohol and chloroform. Phytochemical analysis of neem azadirachta indicaphytochemical constituents ethanol methanol.
The results showed that both leaves contain alkaloids, flavonoids, tannins, saponins, glycosides, steroids and phenolic compounds. Phytochemical extraction and antimicrobial properties of. Neem seeds are used in traditional medicine to treat infections conditions especially those involving the eye and ear. The present study includes the phytochemical detection and antimicrobial activity of the leaves of azadirachta indica, a.
Phytochemical profiling of phytoconstituents of grape. The qualitative phytochemical screening and gcms analysis of ocimum sanctum leaves extracts is discussed by devendran and balasubramanian 8. Phytochemical analysis and antibacterial properties of azadirachta indica neem leaves extract against li article pdf available january 2018 with 99 reads how we measure reads. Phytochemical screening was carried out on extract by. Preliminary phytochemical analysis of the extracts of. Quantitative phytochemical screenings were done to determine the amounts of phytochemical. General techniques involved in phytochemical analysis. The seed of neem azadirachta indica meliaceae was analyzed by various chromatographic methods. Phytochemical and proximate analyses of psidium guajava. Phytochemical analysis of methanolic extracts of leaves of some medicinal plants sudipa nag, anirban paul and rituparna dutta department of botany, rampurhat college, rampurhat, birbhum, west bengal, india. Neem is an ever green tree which grows well in all types of soils. Qualitative phytochemical analysis demonstrated the presence of tannins and saponins in all plants tested. Biochemical analysis includes the estimation of chlorophyll content, carbohydrate content and proline content. The aim of the present study was to investigate the phytochemical screening and in vitro antioxidant activity of aqueous and ethanolic leaf extract of hibiscus rosasinensis and azadirachta indica.
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